Product Includes
Application Dilution
Immunofluorescence 1:200–1:300
Immunohistochemistry 1:100
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
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Reviews
IR57-195 anti-Occludin antibody WB image
Anti- Occludin antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR57-195 overnight at 4°C.
IR57-195 anti-Occludin antibody WB image
Anti- Occludin antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR57-195 overnight at 4°C.
Customer feedback Image IR57-195 anti-Occludin antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with IR57-195 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Customer feedback Image IR57-195 anti-Occludin antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Occlidin with IR57-195 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0