Product Includes
Application Dilution
Immunofluorescence 1:200–1:400
Immunohistochemistry (Frozen section)1:100
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
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Reviews
IR57-193 anti-Occludin antibody WB image
All lanes : Anti- Occludin antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR57-193 overnight at 4°C.
Customer feedback Image IR57-193 anti-Occludin antibody IHC-Fr image
Occludin antibody by Frozen-section
Sample: Rat brain.
Green: Occludin antibody diluted at 1:100.
Blue: DAPI
IR57-193 anti-Occludin antibody WB image
All lanes : Anti- Occludin antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR57-193 overnight at 4°C.
IR57-193 anti-Occludin antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR57-193 anti-Occludin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.