Product Includes
Application Dilution
Immunofluorescence 1:200-1:400
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a heavy chain of an axonemal dynein involved in sperm and respiratory cilia motility. Axonemal dyneins generate force through hydrolysis of ATP and binding to microtubules. [provided by RefSeq, Jan 2012]
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Application : Western blot
Sample : Huh7
Data from : Polaris Pharmaceuticals, Inc.
IR24-12 anti-Na/K ATPase antibody WB image
Anti- Na/K ATPase antibody at 1/700 dilution
Lysates/proteins at 40 μg per lane.
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR24-12 overnight at 4°C
IR24-12 anti-Na/K ATPase antibody ICC/IF image
Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR24-12 anti-Na/K ATPase antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR24-12 anti-Na/K ATPase antibody IHC image
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling Na/K ATPase with IR24-12 at 1/100.