Product Includes
Application Dilution
Immunofluorescence 1:100-1:300
Immunohistochemistry (Paraffin) 1:100-1:300
Immunohistochemistry (Frozen) 1:100-1:300
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
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Reactivity Mouse
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Reactivity Human
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Reactivity
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Reviews
IR13-36 anti-Iba1/AIF1 antibody_Mouse Brain IHC image
Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling Iba1 with IR13-36 at 1/200.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR13-36 anti-Iba1/AIF1 antibody_Rat Brain IHC image
Immunohistochemical analysis of paraffin embedded Rat brain tissue labeling Iba1 with IR13-36 at 1/200.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR13-36 anti-Iba1/AIF1 antibody_Colorectal carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with IR13-36 at 1/200.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR13-36 anti-Iba1/AIF1 antibody_Lymoph node carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with IR13-36 at 1/200.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR13-36 anti-Iba1/AIF1 antibody WB image
Iba1/AIF1 antibody - at 1/500 dilution
Lysates at 45 μg per lane, This blot was produced using a 15% SDS-PAGE. PVDF membrane was then blocked with 3%BSA for an hour before being incubated with IR13-36 overnight at 4°C.
Customer feedback Image IR13-36 anti-Iba1/AIF1 antibody IHC-Fr image
Frozen sectioned for immunohistochemical analysis.
Sample: Frozen sectioned Rat brain.
Green: Iba1 antibody (IR13-36)
Red: TUJ1 antibody
Blue: DAPI
Customer feedback Image IR13-36 anti-Iba1/AIF1 antibody IHC-Fr image
Immunohistochemical of frozen sections.
Sample : mouse cerebellum.
Green : Iba1 antibody (IR13-36) : 1-200
Anti-rabbit 488 : 1-500.
DAPI (blue) was used as the nuclear counter stain.
Customer feedback Image IR13-36 anti-Iba1/AIF1 antibody IHC-Fr image
Immunohistochemical of frozen sections.
Sample : mouse hippocampus.
Green : Iba1 antibody (IR13-36) : 1-200
Anti-rabbit 488 : 1-500.
DAPI (blue) was used as the nuclear counter stain.