Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:500
Immunohistochemistry (Paraffin) 1:100 – 1:200
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
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Application : Western blot
Condition / Dilution： 1-1000, RT 2hr
Sample : Neuro 2A
Data from : Taipei Medical University
IR8-23 anti-Nestin antibody WB image
Anti-Nestin antibody at 1/1000 dilution
Lysates at 30 μg per lane.
IR8-23 anti-Nestin antibody WB image
Sample : Neuron 2A
Nestin antibody (IR8-23) : 1-1000
IR8-23 anti-Nestin antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with IR8-23 at 1/100.
IR8-23 anti-Nestin antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with IR8-23 at 1/100.
IR8-23 anti-Nestin antibody_Hunan brain IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with IR8-23 at 1/100.
IR8-23 anti- Nestin antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR8-23 anti-Nestin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.