Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
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Application : Western blot
Condition / Dilution： 1-1000, RT 2hr
Sample : Neuro 2A
Data from : Taipei Medical University
IR5-11 anti-Lamin B1 antibody WB image
All lanes : Anti-Lamin B1 antibody - at 1/2000 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR5-11 overnight at 4°C.
IR5-11 anti-Lamin B1 antibody WB image
A549 cells'cytoplasmic and nuclear proteins extracted by 30-min.
A. SDS-PAGE gel shows the profiles of extracted cytoplasmic proteins and nuclear proteins.
B. The proteins from the gel were transferred to nitrocellulose membrane and probed with Lamin B1 antibody, Histone H3 antibody (nuclear marker) and GAPDH antibody (cytoplasmic marker).
Nuclear Protein Extraction Kit (Cat.# IR20107).
IR5-11 anti-Lamin B1 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR5-11 anti-Lamin B1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.