Product Includes
Application Dilution
Immunofluorescence 1:500 – 1:1000
Immunohistochemistry (Paraffin) 1:100 – 1:300
Immunohistochemistry (Frozen) 1:100 – 1:300
Flow Cytometry 1:50 – 1:150
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
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Reviews
IR75-254 CD31 (human specific) antibody WB image
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR75-245 overnight at 4°C.
Anti- CD31 (human specific) antibody - at 1/1000 dilution
Lysates at 35 μg per lane.
IR75-254 CD31 (human specific) antibody IHC image
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD31 with IR75-254 at 1/150.
IR75-254 CD31 (human specific) antibody IHC image
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling CD31 with IR75-254 at 1/150
IR75-254 anti-CD31 (human specific) antibody IHC-Fr image
Immunohistochemical of frozen sections.
Sample : Human tissue
CD31 antibody : 1-200
Blue : DAPI was used as the nuclear counter stain.
IR75-254 CD31 (human specific) antibody FACS image
Human peripheral blood granulocytes were stained with IR75-254, CD31 antibody (human specific).