Product Includes
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Application Dilution
Western Blot 1:500 – 1:1000
Immunofluorescence 1:100 – 1:400
Immunohistochemistry (Paraffin) 1:100
Immunohistochemistry (Frozen) 1:100 – 1:200
Immunofluorescence 1:100 – 1:400
Immunohistochemistry (Paraffin) 1:100
Immunohistochemistry (Frozen) 1:100 – 1:200
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Endogenous
Source / Immunogen
Synthetic peptide /S100 calcium binding protein B
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IR27-69 anti-S100B antibody WB image
This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR27-69 overnight at 4°C.
Lysates/proteins at 35 μg per lane
Anti-S100B antibody at 1/700 dilution
IR27-69 anti-S100B antibody IHC image
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling S100B antibody with IR27-69 at 1/100.
IR27-69 anti-S100B antibody IHC-Fr image
Immunohistochemical of frozen sections.
Sample : rat brain.
Green : S100B (IR27-69) : 1-200
Anti-rabbit 488 : 1-500