Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:300
Immunohistochemistry (Paraffin) 1:100 – 1:200
Storage
Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]
IRM012 ApoE / Apolipoprotein E monoclonal antibody WB image
Anti-ApoE / Apolipoprotein E monoclonal antibody at 1/800 dilution. Lysates at 30 μg per lane. NC membrane was then blocked for an hour before being incubated with IRM012 overnight at 4°C.
IRM012 ApoE / Apolipoprotein E monoclonal antibody ICC/IF image
HepG2 cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IRM012 anti- ApoE / Apolipoprotein E monoclonal antibody (red) at 1:200 and 4°C.
IRM012 ApoE / Apolipoprotein E monoclonal antibody IHC image
Immunohistochemical analysis of paraffin embedded Human tissue labeling ApoE with IRM012 at 1/100.