Product Includes
Application Dilution
Immunofluorescence 1:100 – 1:300
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]
IR353-1 anti-ASPM antibody WB image
Anti-ASPM antibody at 1/500 dilution
Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR353-1 overnight at 4°C.
IR353-1 anti-ASPM antibody ICC image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR353-1 anti-ASPM antibody (red) at 1:200 and 4°C.
IR353-1 anti-ASPM antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ASPM with IR353-1 at 1/100.