Product Includes
Application Dilution
Immunofluorescence 1:100-1:300
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]
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Reviews
IR72-249 anti-TIMP1 antibody WB image
Anti-TIMP1 antibody at 1/1000 dilution.
Lysates at 40 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR72-249 overnight at 4°C.
IR72-249 anti-TIMP1 antibody WB image
Anti-TIMP1 antibody at 1/1000 dilution.
Lysates at 40 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR72-249 overnight at 4°C.
IR72-249 anti-TIMP1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling TIMP1 with IR72-249 at 1/100.
IR72-249 anti-TIMP1 antibody_HT-29 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR72-249 anti- TIMP1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR72-249 anti-TIMP1 antibody_MDA-MB-231 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR72-249 anti- TIMP1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.