Product Includes
Application Dilution
Immunofluorescence 1:150-1:300
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
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Reviews
IR71-245 anti-MMP14 / MT1-MMP antibody WB image
All lanes : Anti-MMP14 / MT1-MMP antibody - Loading Control at 1/500 dilution
Lysates/proteins at 30 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR71-245 overnight at 4°C.
IR71-245 anti-MMP14 / MT1-MMP antibody WB image
All lanes : Anti-MMP14 / MT1-MMP antibody - Loading Control at 1/500 dilution
Lysates/proteins at 30 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR71-245 overnight at 4°C.
IR71-245 anti- MMP14 / MT1-MMP antibody_Breast carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling MMP14 / MT1-MMP with IR71-245 at 1/100.
IR71-245 anti-MMP14 / MT1-MMP antibody IHC image
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Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14 / MT1-MMP with IR71-245 at 1/100.
IR71-245 anti-MMP14 / MT1-MMP antibody IHC image
Customer feedback Image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14 / MT1-MMP with IR71-245 at 1/100.
IR71-245 anti- MMP14 / MT1-MMP antibody_Huh-7 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR71-245 anti-MMP14 / MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR71-245 anti- MMP14 / MT1-MMP antibody_HT-29 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR71-245 anti-MMP14 / MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.