Product Includes
Application Dilution
Immunofluorescence 1:200–1:400
Immunohistochemistry (Paraffin) 1:100-1:150
Flow Cytometry 1:50-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Reviews
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Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : EpCAM antibody (extracellular domain) (IR218-3)
Data from : Nebulum Technologies Co., Ltd.
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Application : FFPE immunohistochemistry
Condition / Dilution : 1-200
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Sample : mouse colon
Data from : Li-Tzung Biotechnology. Co
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Application : Western blot
Condition / Dilution : 1-2000
Sample : HCT-116 drug treatment
Data from : Taipei Medical University
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Application : Western blot
Condition / Dilution : 1-2000
Sample : HCT-116 drug treatment
Data from : Taipei Medical University
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Application : ICC/IF
Condition / Dilution 1-200
Sample : HT-29
Data from : National Yang Ming Chiao Tung University
IR218-3 anti-EpCAM antibody WB image
Anti-EpCAM antibody - at 1/1000 dilution
Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR218-3 overnight at 4°C.
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : EpCAM antibody (extracellular domain) (IR218-3)
Data from : Nebulum Technologies Co., Ltd.
IR218-3 anti- EpCAM antibody IHC image
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr.
IR218-3 anti- EpCAM antibody IHC image
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr.
IR218-3 anti- EpCAM antibody FACS image
Product：EpCAM antibody
Catalog：IR218-3
Cell : MCF-7
IR218-3 anti- EpCAM antibody ICC/IF image
Cell : MCF-7 (DAPI : 1-300, Phalloidin : 1-300)
EpCAM, (IR218-3) : 1-200
Anti-rabbit 594 : 1-500
NikonEpi-fluorescence (A-ken) 60x
IR218-3 anti-EpCAM antibody WB image
Anti-EpCAM antibody - at 1/1000 dilution
Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR218-3 overnight at 4°C.
IR218-3 anti- EpCAM antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR218-3 anti- EpCAM antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0