Product Includes
Application Dilution
Immunofluorescence 1:200–1:300
Immunohistochemistry (Paraffin) 1:200-1:400
Storage
100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]
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Application : Western blot
Condition / Dilution： 1-1000
Sample : H1299, BT-549
Data from : China Medical University
IR132-533 anti-ATF6 antibody WB image
All lanes : Anti-ATF6 antibody - at 1/1000 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR132-533 overnight at 4°C.
IR132-533 anti-ATF6 antibody WB image
All lanes : Anti-ATF6 antibody - at 1/1000 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR132-533 overnight at 4°C.
IR132-533 anti-ATF6 antibody WB image
All lanes : Anti-ATF6 antibody - at 1/1000 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR132-533 overnight at 4°C.
IR132-533 anti-ATF6 antibody WB image
All lanes : Anti-ATF6 antibody - at 1/1000 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR132-533 overnight at 4°C.
IR132-533 anti-ATF6 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR132-533 anti- ATF6 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR132-533 anti-ATF6 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ATF6 with IR132-533 at 1/200.