Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1:100–1:200
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
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Application
Reactivity Human
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Application
Reactivity Human
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Application
Reactivity Mouse
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Application
Reactivity Human
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Application
Reactivity Human
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Reviews
IR116-289 anti-Cyclin B1 antibody WB image
Cyclin B1 antibody - at 1/500 dilution
Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR116-289 overnight at 4°C.
IR116-289 anti-Cyclin B1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling Cyclin B1 with IR116-289 at 1/100.
IR116-289 anti-Cyclin B1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Cyclin B1 with IR116-289 at 1/100.
IR116-289 anti-Cyclin B1 antibody ICC/IF image
Huh-7 cell were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR116-289 anti- Cyclin B1 antibody (red) at 1:400 and 4°C.