Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]
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Application : Western blot
Condition / Dilution： 1-1000, BSA blocking
Sample : HT-29 drug treatment
Data from : Taipei Medical University
IR115-286 anti-Cyclin A2 antibody WB image
All lanes : Anti-Cyclin A2 antibody - Loading Control at 1/1000 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR115-286 overnight at 4°C.
IR115-286 anti-Cyclin A2 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR115-286 anti-Cyclin A2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR115-286 anti-Cyclin A2 antibody WB image
All lanes : Anti-Cyclin A2 antibody - Loading Control at 1/1000 dilution
Lysates/proteins at 30 μg per lane
This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR115-286 overnight at 4°C.
IR115-286 anti-Cyclin A2 antibody_Gastric carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Gastric cancer tissue labeling Cyclin A2 with IR115-286 at 1/100.