Product Includes
Application Dilution
Immunofluorescence 1:200-1:300
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]
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Reviews
IR106-P2 anti-TNF alpha antibody WB image
Anti- TNF alpha antibody at 1/700 dilution
Lysates at 50 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR106-P2 overnight at 4°C.
IR106-P2 anti-TNF alpha antibody WB image
Anti- TNF alpha antibody at 1/700 dilution
Lysates at 50 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR106-P2 overnight at 4°C.
IR106-P2 anti-TNF alpha antibody_Liver carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling TNF alpha with IR106-P2 at 1/150.
IR106-P2 anti-TNF alpha antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR106-P2 anti- TNF alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.