Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Reviews
IR78-268 anti-ICAM-1 / CD54 antibody_HT-29 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR78-268 anti- ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR78-268 anti-ICAM-1 / CD54 antibody WB image
ICAM-1 / CD54 antibody - at 1/500 dilution
Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR78-268 overnight at 4°C.
IR78-268 anti-ICAM-1 / CD54 antibody_MDA-MB-231 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR78-268 anti- ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR78-268 anti-ICAM-1 / CD54 antibody WB image
ICAM-1 / CD54 antibody - at 1/500 dilution
Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR78-268 overnight at 4°C.
IR78-268 anti-ICAM-1 / CD54 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ICAM-1 with IR786-268 at 1/100.
IR78-268 anti-ICAM-1 / CD54 antibody WB image
ICAM-1 / CD54 antibody - at 1/500 dilution
Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR78-268 overnight at 4°C.