Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1: 200-1:400
Flow Cytometry 1:50-1:100
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
| Title | |
|---|---|
| No data. | |
Reviews
IR77-264 anti-CD45 antibody WB image
All lanes : Anti-CD45 antibody at 1/1000 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR77-264 overnight at 4°C.
IR77-264 anti-CD45 antibody FACS image
Product：CD45 antibody
Catalog：IR77-264
Cell : THP-1
IR77-264 anti- CD45 antibody_Colorectal carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling CD45 with IR77-264 at 1/300.
IR77-264 anti-CD45 antibody_THP-1 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR77-264 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR77-264 anti-CD45 antibody_WBC ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR77-264 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR77-264 anti- CD45 antibody_Breast carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with IR77-264 at 1/300.
IR77-264 anti- CD45 antibody_Liver carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with IR77-264 at 1/300.
IR77-264 anti- CD45 antibody_Lung carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with IR77-264 at 1/300.
IR77-264 anti- CD45 antibody_Lymph node carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Lymph node carcinoma labeling CD45 with IR77-264 at 1/300.
IR77-264 anti- CD45 antibody_Squamous cell carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Squamous cell carcinoma labeling CD45 with IR77-264 at 1/300.
IR77-264 anti- CD45 antibody_Tonsil carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling CD45 with IR77-264 at 1/300.
IR77-264 anti- CD45 antibody_Ovary carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Ovary cancer tissue labeling CD45 with IR77-264 at 1/300.
IR77-264 anti- CD45 antibody_Gastric carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Gastric cancer tissue labeling CD45 with IR77-264 at 1/300.