Product Includes
Application Dilution
Immunofluorescence 1:200-1:400
Immunohistochemistry (Paraffin) 1:100-1:300
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]
Reviews
IR50-154 anti-SNAIL antibody WB image
All lanes : Anti-SNAIL antibody at 1/1000 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR50-154 overnight at 4°C.
IR50-154 anti-SNAIL antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling SNAIL with IR50-154 at 1/300.
IR50-154 anti-SNAIL antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling SNAIL with IR50-154 at 1/300.
IR50-154 anti-SNAIL antibody WB image
All lanes : Anti-SNAIL antibody at 1/3000 dilution
Lysates/proteins at 35 μg per lane
This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR50-154 overnight at 4°C.
IR50-154 anti-SNAIL antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR50-154 anti-SNAIL antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.