Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1:100-1:400
Immunohistochemistry (Frozen) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]
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Reviews
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : α Smooth Muscle Actin antibody (IR47-146)
Data from : Nebulum Technologies Co., Ltd.
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Application : FFPE immunohistochemistry
Condition / Dilution： 1-200
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Sample : mouse colon
Data from : Li-Tzung Biotechnology. Co
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Application : IF / Frozen section
Data from : Fu Jen Catholic University Hospital
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Application : FFPE immunohistochemistry
Condition / Dilution ：1-200
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Data from : Li-Tzung Biotechnology. Co
IR47-146 anti-α Smooth Muscle Actin antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with IR47-146 at 1/300.
IR47-146 anti-α Smooth Muscle Actin antibody WB image
Anti-α Smooth Muscle Actin antibody at 1/2000 dilution
Lysates at 35 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR47-146 overnight at 4°C.
IR47-146 anti-α Smooth Muscle Actin antibody_Colorectal carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with IR47-146 at 1/200.
IR47-146 anti-α Smooth Muscle Actin antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with IR47-146 at 1/300.
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : α Smooth Muscle Actin antibody (IR47-146)
Data from : Nebulum Technologies Co., Ltd.
Customer feedback Image IR47-146 anti-α Smooth Muscle Actin antibody IHC image
Frozen sectioned for immunohistochemical analysis.
Sample: Frozen section.
Red: α Smooth Muscle Actin antibody.
Catalog: IR47-146 at 1-200
IR47-146 anti-α Smooth Muscle Actin antibody_Liver carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with IR47-146 at 1/200.
IR47-146 anti-α Smooth Muscle Actin antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with IR47-146 at 1/300.
IR47-146 anti-α Smooth Muscle Actin antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR47-146 anti- α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR47-146 anti-α Smooth Muscle Actin antibody_Kidney carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with IR47-146 at 1/200.
IR47-146 anti-α Smooth Muscle Actin antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR47-146 anti- α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.