Product Includes
Application Dilution
Immunofluorescence 1:200-1:300
Immunohistochemistry (Frozen) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
| Title | |
|---|---|
| No data. | |
Reviews
IR43-128 anti-alpha Synuclein antibody WB image
Anti-alpha Synuclein / SNCA antibody - 1/700 dilution
Lysates at 60 μg per lane
This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR43-128 overnight at 4°C.
Customer feedback Image IR43-128 anti-alpha Synuclein antibody IF image
Immunofluorescent analysis.
Sample: primary cortical neurons
alpha Synuclein (IR43-128) : 1-400
Anti-rabbit 488: 1-500
Fixed: 4% paraformaldehyde at RT for 20 min.
Customer feedback Image IR43-128 anti-alpha Synuclein antibody IHC image
Immunohistochemical of frozen sections.
sample : mouse cerebellum.
alpha Synuclein (IR43-128) : 1-400
Anti-rabbit 488 : 1-500
IR43-128 anti-alpha Synuclein antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR43-128 anti-alpha Synuclein antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.