Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:500
Immunohistochemistry (Paraffin) 1:200 – 1:300
Immunohistochemistry (Frozen) 1:200 – 1:400
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
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Application : Western blot
Condition / Dilution： 1-2000
Data from : Chang Gung University
IR39-123 anti-Tau antibody WB image
Anti-Tau antibody
Lysates at 40 μg per lane.
This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR39-123 overnight at 4°C.
IR39-123 anti-Tau antibody WB image
All lanes : Anti-Tau antibody
Lysates/proteins at 45 μg per lane.
This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR39-123 overnight at 4°C.
IR39-123 anti-Tau antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse Alzheimer’s tissue labeling Tau with IR39-123 at 1/1000 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Data from: Li-Tzung Biotechnology. Co
IR39-123 anti-Tau antibody IHC image
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Tau with IR39-123 at 1/200.
IR39-123 anti-Tau antibody IHC image
Immunohistochemical analysis of paraffin embedded Human astroglioma tissue labeling Tau with IR39-123 at 1/300.
IR39-123 anti-Tau antibody IHC image
Immunohistochemical of frozen sections.
Sample : mouse cerebellum.
Green : Tau antibody (IR39-123) : 1-300
Anti-rabbit 488 : 1-500
DAPI (blue) was used as the nuclear counter stain.
IR39-123 anti-Tau antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR39-123 anti-Tau antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR39-123 anti-Tau antibody IF image
Immunofluorescent analysis.
Sample : primary cortical neurons
Tau antibody (IR39-123) : 1-300
Anti-rabbit 488 : 1-500
Fixed : 4% paraformaldehyde at RT for 20 min.
IR39-123 anti-Tau antibody IF image
Immunofluorescent analysis.
Sample : primary cortical neurons
Tau antibody (IR39-123) : 1-300
Anti-rabbit 488 : 1-500
Fixed : 4% paraformaldehyde at RT for 20 min.