CD133 / Prominin-1 antibody (Intracellular domain)
Product Includes
Application Dilution
Immunofluorescence 1:300-1:500
Immunohistochemistry (Paraffin) 1:200-1:400
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
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Application : Western blot
Condition / Dilution：1-500, BSA blocking
Sample : HT-29 drug treatment
Data from : Taipei Medical University
IR30-79 anti- CD133 / Prominin-1 antibody WB image
All lanes : Anti-CD133 antibody at 1/700 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR30-79 overnight at 4°C.
IR30-79 anti- CD133 / Prominin-1 antibody WB image
All lanes : Anti-CD133 antibody at 1/700 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR30-79 overnight at 4°C.
IR30-79 anti- CD133 / Prominin-1 antibody WB image
All lanes : Anti-CD133 antibody at 1/700 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR30-79 overnight at 4°C.
IR30-79 anti- CD133 / Prominin-1 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR30-79 anti- CD133 / Prominin-1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR30-79 anti- CD133 / Prominin-1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD133 / Prominin-1 with IR30-79 at 1/200.
IR30-79 anti- CD133 / Prominin-1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD133 / Prominin-1 with IR30-79 at 1/200.