Product Includes
Application Dilution
Immunofluorescence 1:200-1:400
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
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Reviews
IR20-56 anti-NeuN antibody IHC image
Immunohistochemical analysis of paraffin embedded Human Brain labeling NeuN with IR20-56 at 1/100.
IR20-56 anti-NeuN antibody IHC image
Immunohistochemical analysis of paraffin embedded Human Brain labeling NeuN with IR20-56 at 1/100.
IR20-56 anti-NeuN antibody WB image
Anti-NeuN antibody at 1/1000 dilution
Lysates at 50 μg per lane.
IR20-56 anti-NeuN antibody IF image
Sample: rat primary cortical neurons cells were fixed in 4% PFA at RT for 15 min.
Green: Tuj1 antibody
Red: NeuN antibody (IR20-56) diluted at 1:200.
IR20-56 anti-NeuN antibody IF image
Sample: rat primary cortical neurons cells were fixed in 4% PFA at RT for 15 min.
Green: NeuN antibody (IR20-56) diluted at 1:200.