Product Includes
Application Dilution
Immunofluorescence 1:200-1:300
Immunohistochemistry (Paraffin) 1:100-1:150
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
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Reviews
Customer feedback Image
Application : Western blot
Condition / Dilution： 1-5000, RT 2hr
Sample : Neuro 2A
Data from : Taipei Medical University
Customer feedback Image
Application : Immunofluorescence Staining
Sample : iPS-derived neuron progenitor cells (p4)
Data from : National Yang Ming Chiao Tung University
Customer feedback Image IR12-33 anti-MAP2 antibody IF image
Immunofluorescent analysis.
Sample : primary cortical neurons
MAP2 antibody (IR12-33) : 1-200
Anti-rabbit 488 : 1-500
Fixed : 4% paraformaldehyde at RT for 20 min.
IR12-33 anti-MAP2 antibody_Human brain IHC image
Immunohistochemical analysis of paraffin embedded Human brain and mouse tissue labeling MAP2 antibody with IR12-33 at 1/100.
IR12-33 anti-MAP2 antibody_Mouse brain IHC image
Immunohistochemical analysis of paraffin embedded Human brain and mouse tissue labeling MAP2 antibody with IR12-33 at 1/100.
IR12-33 anti-MAP2 antibody ICC image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR12-33 anti-MAP2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR12-33 anti-MAP2 antibody WB image
All lanes : Anti-MAP2 antibody at 1/500 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR12-33 overnight at 4°C.