Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1:100-1:300
Immunohistochemistry (Frozen) 1:100-1:300
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
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Reviews
IR11-31 anti-Tuj1 antibody IHC image
Immunohistochemical analysis (Frozen sectioned)
Sample: Rat brain
Red: Tuj1 antibody (IR11-31)
Blue: DAPI
IR11-31 anti-Tuj1 antibody IF image
Immunofluorescent analysis.
Sample : primary cortical neurons
Tuj1 / Beta Tubulin 3 (IR11-31) : 1-300
Anti-rabbit 488 : 1-500
Fixed : 4% paraformaldehyde at RT for 20 min.
IR11-31 anti-Tuj1 antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR11-31 anti-Tuj1 antibody (red) at 1:200 and 4°C.
IR11-31 anti- Beta Tubulin 3/ Tuj1 antibody WB image
All lanes : Anti- Beta Tubulin 3/ Tuj1 antibody -at 1/5000 dilution
Lysates/proteins at 20 μg per lane
This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR11-31 overnight at 4°C.
IR11-31 anti- Beta Tubulin 3/ Tuj1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tuj1 with IR11-31 at 1/200.