| REACTIVITY | Human |
|---|---|
| SENSITIVITY | - |
| MW | 40-50, 55-7 |
| SOURCE | Rabbit-IgG |
Product Includes
-
Application Dilution
Western Blot 1-1000
Immunohistochemistry (Paraffin) 1:100 – 1:200
Immunohistochemistry (Paraffin) 1:100 – 1:200
Storage
Long time storage is recommended at -20°C
100mM Tris Glycine, 30% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
100mM Tris Glycine, 30% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
-
Source / Immunogen
Polyclonal antibodies are produced by immunizing animals with a recombinant corresponding to residues near the amino terminus of human Nectin-4/PVRL4 protein.
-
IR283-P1 anti Nectin-4 / PVRL4 antibody WB image
This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR283-P1 overnight at 4°C.
Anti Nectin-4 / PVRL4 antibody - at 1/1000 dilution
Lysates/proteins at 25 μg per lane
IR283-P1 anti Nectin-4 / PVRL4 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling Nectin-4 / PVRL4 with IR283-P1 at 1/200.