- Following reaction with peroxidase, a blue reaction product forms that may be read at 370 nm or between 620 and 655 nm. For end-point assays, the reaction can be stopped by the addition of a volume of 1M or 2M HCl, or 0.5M H2SO4, equal to the volume of the substrate reaction in the well. The resulting yellow end-product, which is stable for at least one hour, can then be read at 450 nm. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted. For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Real TMB ELISA Substrate | Content | Package |
---|---|---|
IRRG8005-100ML | Real TMB ELISA Substrate |
100ml |
IRRG8005-500ML | Real TMB ELISA Substrate | 500ml |