Exosome
CD31
Antibodies Panel
TMB Substrate TMB Substrate
Catalog Number:IRRG8005

Real TMB ELISA Substrate

Application
  • ELISA
Reactivity -

REACTIVITY -
SENSITIVITY -
MW -
SOURCE -

Product Includes

  • Following reaction with peroxidase, a blue reaction product forms that may be read at 370 nm or between 620 and 655 nm. For end-point assays, the reaction can be stopped by the addition of a volume of 1M or 2M HCl, or 0.5M H2SO4, equal to the volume of the substrate reaction in the well. The resulting yellow end-product, which is stable for at least one hour, can then be read at 450 nm. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted. For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Real TMB ELISA Substrate Content Package
IRRG8005-100ML Real TMB ELISA Substrate
100ml
IRRG8005-500ML Real TMB ELISA Substrate 500ml

Application Dilution

-

Storage

Shipped at 4℃. Upon delivery aliquot and store at 2℃ or 8℃.
Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures

Specificity / Sensitivity

-

Source / Immunogen

-

TMB ELISA Substrate detects horseradish peroxidase (HRP) activity and is supplied as a ready to use solution. The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm, or addition of stop solution changes the color to yellow, enabling accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

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