Heme Oxygenase 1 / HMOX1, monoclonal antibody
| REACTIVITY | Human |
|---|---|
| SENSITIVITY | Endogenous |
| MW | 30 |
| SOURCE | Mouse IgG1 |
Product Includes
Application Dilution
Immunofluorescence 1:100 – 1:200
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
IRM044 Heme Oxygenase 1 / HMOX1, monoclonal antibody WB image
Lysates/ at 35 μg per lane.
Heme Oxygenase 1 / HMOX1, monoclonal antibody at 1/500 dilution.
Nitrocellulose membrane was then blocked for an hour before being incubated with IRM044 overnight at 4°C.
IRM044 Heme Oxygenase 1 / HMOX1, monoclonal antibody ICC/IF image
Immunofluorescence of cell.
Sample: HepG2 cells (500uM cocl2-treated)
Green: Heme Oxygenase 1 / HMOX1, monoclonal antibody (IRM044): 1-200
IRM044 Heme Oxygenase 1 / HMOX1, monoclonal antibody IHC image
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue.