Product Includes
-
Application Dilution
Western Blot 1:500 – 1:1000
Immunofluorescence 1:100 – 1:200
Immunohistochemistry (Paraffin) 1:50 – 1:100
Immunofluorescence 1:100 – 1:200
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C
Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Endogenous
Source / Immunogen
-
-
IRM033 NF-kB p65, monoclonal antibody WB image
NF-kB p65, monoclonal antibody at 1/500 dilution. Lysates/ at 35 μg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with IRM033 overnight at 4°C.
IRM033 NF-kB p65, monoclonal antibody ICC/IF image
Immunofluorescence of cell.
Sample: A431 cells
Green: NF-kB p65, monoclonal antibody
(IRM033): 1-200
IRM033 NF-kB p65, monoclonal antibody IHC image
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue.