Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1:200-1:400
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
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Application
Reactivity Human
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Reviews
IR105-435 anti-Cytochrome C antibody WB image
All lanes : Anti- Cytochrome C antibody at 1/1000 dilution
Lysates/proteins at 45 μg per lane.
This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR105-435 overnight at 4°C.
IR105-435 anti-Cytochrome C antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytochrome C with IR105-435 at 1/200.
IR105-435 anti-Cytochrome C antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR105-435 anti- Cytochrome C antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR105-435 anti-Cytochrome C antibody IHC image
Immunohistochemical analysis of paraffin embedded tissue labeling Cytochrome C with IR105-435 at 1/300.