ME1 / malic enzyme 1, monoclonal antibody
Product Includes
Application Dilution
Immunofluorescence 1:100 – 1:200
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
Phosphate-buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
IRM025 ME1 / malic enzyme 1, monoclonal antibody WB image
IRM025 ME1 / malic enzyme 1, monoclonal antibody at 1/500 dilution. Lysates/ at 35 μg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with IRM025 overnight at 4°C.
IRM025 ME1 / malic enzyme 1, monoclonal antibody ICC/IF image
Immunofluorescence of cell.
Sample: HeLa
Green: ME1 / malic enzyme 1, monoclonal antibody
(IRM025): 1-100