Product Includes
Application Dilution
Immunofluorescence 1:100 – 1:200
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
Phosphate-buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
IRM028 Hexokinase II / HK2, monoclonal antibody WB image
Nitrocellulose membrane was then blocked for an hour before being incubated with IRM028 overnight at 4°C.
Hexokinase II / HK2 monoclonal antibody at 1/500 dilution. Lysates/ at 35 μg per lane.
IRM028 Hexokinase II / HK2, monoclonal antibody IHC image
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue.
IRM028 Hexokinase II / HK2, monoclonal antibody ICC/IF image
Immunofluorescence of cell.
Sample: HeLa
Green: Hexokinase II / HK2 antibody
(IRM028): 1-100