Product Includes
Application Dilution
Immunofluorescence 1:100 – 1:200
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
Phosphate-buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
IRM024 NOX2 gp91phox, monoclonal antibody IHC image
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue.
IRM024 NOX2 gp91phox, monoclonal antibody WB image
Lysates/ at 40 μg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with IRM024 overnight at 4°C.
NOX2 gp91phox, monoclonal antibody at 1/500 dilution.