Product Includes
Application Dilution
Immunofluorescence 1:300-1:400
Immunohistochemistry (Paraffin) 1:100-1:300
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
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Reactivity Human
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Reactivity Mice
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Reactivity Human
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Reactivity Human
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Reviews
IR100-414 anti-Caspase 9 cleaved Asp315 antibody WB image
All lanes : Anti-Caspase 9 cleaved Asp315 antibody - at 1/500 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR100-414 overnight at 4°C.
IR100-414 anti-Caspase 9 cleaved Asp315 antibody WB image
All lanes : Anti-Caspase 9 cleaved Asp315 antibody - at 1/500 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR100-414 overnight at 4°C.
IR100-414 anti-Caspase 9 cleaved Asp315 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR100-414 anti- Caspase 9 cleaved Asp315 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR100-414 anti-Caspase 9 cleaved Asp315 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Caspase 9 cleaved Asp315 with IR100-414 at 1/200.