Product Includes
Application Dilution
Immunofluorescence 1:500 – 1:1000
Immunohistochemistry (Paraffin) 1:400 – 1:800
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
IR357-1 anti-PCNA antibody WB image
This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR357-1 overnight at 4°C.
Lysates/proteins at 35 μg per lane.
Anti-PCNA antibody - at 1/3000 dilution
IR357-1 anti-PCNA antibody IHC image
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling PCNA with IR357-1 at 1/400.
IR357-1 anti-PCNA antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR357-1 anti-PCNA antibody (Green) at 1:500 and 4°C.