Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:500
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
MKI67 (Marker Of Proliferation Ki-67) is a Protein Coding gene. Diseases associated with MKI67 include Hepatocellular Carcinoma and Cervical Intraepithelial Neoplasia. Among its related pathways are DNA Damage and Primary focal segmental glomerulosclerosis (FSGS). Gene Ontology (GO) annotations related to this gene include RNA binding and protein C-terminus binding. An important paralog of this gene is CDCA2.[provided by GeneCards, Jan 2023]
IR212-1 Ki-67 antibody WB image
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR212-1 overnight at 4°C.
Lysates at 35 μg per lane.
Anti Ki-67 antibody - at 1/500 dilution.
IR212-1 Ki-67 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 antibody with IR212-1 at 1/100.
IR212-1 Ki-67 antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR212-1 anti-Ki-67 antibody at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.