![IRM309 c-Myc antibody [PK-9] WB image](/Upload/Products/1673921194_6cb88a74ebcbe69953c8.jpg "IRM309 c-Myc antibody [PK-9] WB image")
Product Includes
Application Dilution
Immunofluorescence 1:300 – 1:400
Storage
Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
IRM309 c-Myc antibody [PK-9] WB image
This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRM309 overnight at 4°C.
Anti-c-Myc antibody [PK-9] at 1/800 dilution
Lysates at 30 μg per lane.