Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1:100
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
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Application
Reactivity
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Application
Reactivity Mouse
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Reviews
Customer feedback Image
Application : Western blot
Condition / Dilution： 1-1000
Sample : SH-SY5Y drug treatment
Data from : Chang Gung University
Customer feedback Image
Application : Western blot
Condition / Dilution：1-1000
Sample : Raw264.7 / LPS treatment
Data from : Yuanpei University of Medical Technology
IR231-856 anti-iNOS antibody WB image
Anti-iNOS antibody - at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with IR231-856 overnight at 4°C.
IR231-856 anti-iNOS antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR231-856 anti iNOS antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR231-856 anti-iNOS antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR231-856 anti iNOS antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR231-856 anti-iNOS antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse testis labeling iNOS with IR231-856 at 1/250.
IR231-856 anti-iNOS antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse testis labeling iNOS with IR231-856 at 1/250.