![IRM202 Integrin β1 / CD29 antibody [C1H1] WB image](/Upload/Products/1667453095_91960ea7e916476b66e2.jpg "IRM202 Integrin β1 / CD29 antibody [C1H1] WB image")
![IRM202 Integrin β1 / CD29 antibody [C1H1] WB image](/Upload/Products/1667290165_bc4f46908695cda152fa.jpg "IRM202 Integrin β1 / CD29 antibody [C1H1] WB image")
![IRM202 Integrin β1 / CD29 antibody [C1H1] IHC image](/Upload/Products/1671776858_85823a5e6c9d0ad81a0c.jpg "IRM202 Integrin β1 / CD29 antibody [C1H1] IHC image")
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Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:500
Immunohistochemistry (Paraffin) 1:50 – 1:100
Storage
Phosphate-buffered solutions, pH 7.2, 50% Glycerol, 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
IRM202 Integrin β1 / CD29 antibody [C1H1] WB image
Anti- Integrin β1 / CD29 antibody [C1H1] at 1/500 dilution
Lysates at 30 μg per lane.
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IRM202 overnight at 4°C.
IRM202 Integrin β1 / CD29 antibody [C1H1] WB image
Anti- Integrin β1 / CD29 antibody [C1H1] at 1/1000 dilution
Lysates at 30 μg per lane.
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRM202 overnight at 4°C.
IRM202 Integrin β1 / CD29 antibody [C1H1] IHC image
Customer feedback Image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Integrin β1 / CD29 with IRM202 at 1/50.
IRM202 Integrin β1 / CD29 antibody [C1H1] ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IRM202 anti- Integrin β1 / CD29 antibody [C1H1] (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.