Product Includes
Application Dilution
Immunofluorescence 1:300 – 1:500
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
IR344-930 anti-PPARγ antibody WB image
Anti-PPARγ antibody at 1/1000 dilution
Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR344-930 overnight at 4°C.
IR344-930 anti-PPARγ antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PPARγ with IR344-930 at 1/100.
IR344-930 anti-PPARγ antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PPARγ with IR344-930 at 1/100.
IR344-930 anti-PPARγ antibody IHC image
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PPARγ with IR344-930 at 1/100.
IR344-930 anti-PPARγ antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR344-930 anti-PPARγantibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR344-930 anti-PPARγ antibody IHC image
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PPARγ with IR344-930 at 1/100.