Catalog Number：IRBS4012
Anti-Rabbit IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
Application
Reactivity Rabbit IgG
Product Includes
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Application Dilution
User Optimized
Western Blot 1:10,000
ELISA 1:20,000–1:40,000
Immunohistochemistry 1:500 – 1:1,000 (Other Assays User Optimized)
Western Blot 1:10,000
ELISA 1:20,000–1:40,000
Immunohistochemistry 1:500 – 1:1,000 (Other Assays User Optimized)
Storage
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA), 50% Glycerol, 0.025% ProClin 300 was added as a preservative, Do NOT add Sodium Azide!
Specificity / Sensitivity
Endogenous
Source / Immunogen
Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemilu
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#IRBS4032
Rat IgG
Anti-Rat IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
#IRBS4022
Mouse IgG
Anti-Mouse IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
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Anti-Rabbit IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
Western Blot of Peroxidase Conjugated Goat Anti-Rabbit IgG Antibody (Catalog. IRBS4012)
Lysates/proteins at 35 μg per lane
The membrane was then blocked for an hour before being incubated with IR3-8 GAPDH antibody overnight at 4°C.
Secondary antibody:
Peroxidase Conjugated Goat Anti-Rabbit IgG Antibody (Catalog. IRBS4012) at 1:10,000 for 60 min at RT.
Predicted/Observed size: 35 kDa.