CD3 (Intracellular domain) antibody
Product Includes
Application Dilution
Immunofluorescence 1:300-1:400
Immunohistochemistry (Paraffin) 1:100-1:300
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Reviews
Customer feedback Image
Application : FFPE immunohistochemistry
Condition / Dilution：1-100
Sample : Mouse colon
Epitope Retrieval methods : Citrate Buffer, pH 6.0
Data from : Li-Tzung Biotechnology. Co
Customer feedback Image
Application : FFPE immunohistochemistry
Antibody : IR252-1 CD3 antibody (Green)
Sample : mouse tissue
Data from : Li-Tzung Biotechnology. Co
IR252-1 anti-CD3 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR252-1 anti-CD3 antibody at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR252-1 anti-CD3 antibody_mouse spleen IHC image
Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with IR252-1 at 1/200 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR252-1 anti-CD3 antibody_mouse spleen IHC image
Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with IR252-1 at 1/200 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR252-1 anti-CD3 antibody_mouse spleen IHC image
Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with IR252-1 at 1/200 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR252-1 anti-CD3 antibody WB image
Anti-CD3 antibody at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with IR252-1 overnight at 4°C.
IR252-1 anti-CD3 antibody FACS image
Product：CD3 antibody
Catalog：IR252-1
Sample : Human peripheral blood
anti-CD3 antibody at 1:100
IR252-1 anti-CD3 antibody_Tonsil carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Tonsil carcinoma tissue labeling CD3 with IR252-1 at 1/200.
IR252-1 anti-CD3 antibody_Hepatocellular carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Hepatocellular cancer tissue labeling CD3 with IR252-1 at 1/100.