Product Includes
Application Dilution
Immunofluorescence 1:100-1:200
Immunohistochemistry (Paraffin) 1:50-1:100
Storage
Phosphate-buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
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Reviews
IRM007 anti-Iba1 monoclonal antibody IHC image
Iba1 monoclonal antibody IRM007. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue.
IRM007 anti-Iba1 monoclonal antibody WB image
Iba1 antibody at 1/500 dilution
Lysates/ at 50 μg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with IRM007 overnight at 4°C.
IRM007 anti-Iba1 monoclonal antibody IF image
Immunohistochemical of frozen sections.
Sample: mouse cerebellum.
Green: Iba1 antibody (IRM007) : 1-200
DAPI (blue) was used as the nuclear counter stain.