Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020]
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Reviews
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Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : EGFR antibody (Extracellular domain), IRST002
Data from : Nebulum Technologies Co., Ltd.
IRST002 anti-EGFR antibody WB image
All lanes : Anti-EGFR antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRST002 overnight at 4°C.
Customer feedback Image
Application : Immunocytochemistry (ICC)
Sample : Mixture cell
Antibody : EGFR antibody (Extracellular domain), IRST002
Data from : Nebulum Technologies Co., Ltd.