Product Includes
Application Dilution
Immunofluorescence 1:200-1:400
Immunohistochemistry (Paraffin) 1:200-1:300
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The human TLR family comprises 11 members. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. For the recognition of structural components in foreign microorganisms, the various TLRs exhibit different patterns of expression as well; in this way for example, TLR-3, -7, and -8 are essential in the recognition of single-stranded RNA viruses. TLR7 senses single-stranded RNA oligonucleotides containing guanosine- and uridine-rich sequences from RNA viruses, a recognition occuring in the endosomes of plasmacytoid dendritic cells and B cells. This gene is predominantly expressed in lung, placenta, and spleen, and is phylogenetically related and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Aug 2020]
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Reviews
IR327-1032 anti- Toll-like Receptor 7 antibody WB image
Anti- Toll-like Receptor 7 antibody at 1/800 dilution
Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR327-1032 overnight at 4°C.
IR327-1032 anti- Toll-like Receptor 7 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse testis labeling Toll-like Receptor 7 with IR327-1032 at 1/200.
IR327-1032 anti- Toll-like Receptor 7 antibody ICC/IF image
Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR327-1032 anti- Toll-like Receptor 7 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.