Product Includes
Application Dilution
Immunofluorescence 1:300-1:500
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It thus plays a role in host defense against multiple viruses. [provided by RefSeq, Jul 2021]
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Reviews
IR323-1016 anti- Toll-like Receptor 3 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR323-1016 anti- Toll-like Receptor 3 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR323-1016 anti- Toll-like Receptor 3 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Toll-like Receptor 3 with IR323-1016 at 1/200.
IR323-1016 anti- Toll-like Receptor 3 antibody WB image
All lanes : Anti- Toll-like Receptor 3 antibody at 1/800 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR323-1016 overnight at 4°C.
IR323-1016 anti- Toll-like Receptor 3 antibody WB image
All lanes : Anti- Toll-like Receptor 3 antibody at 1/800 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR323-1016 overnight at 4°C.
IR323-1016 anti- Toll-like Receptor 3 antibody WB image
All lanes : Anti- Toll-like Receptor 3 antibody at 1/800 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR323-1016 overnight at 4°C.