Product Includes
Application Dilution
Immunofluorescence 1: 100- 1:300
Immunohistochemistry (Paraffin) 1: 100-1:200
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
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Application : Western blot
Condition / Dilution： 1-1000
Sample : HCT-116
Data from : Taipei Medical University
IR66-225 anti-MMP1 antibody WB image
All lanes : Anti-MMP1 antibody at 1/500 dilution
Lysates/proteins at 40 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR66-225 overnight at 4°C.
IR66-225 anti-MMP1 antibody WB image
All lanes : Anti-MMP1 antibody at 1/500 dilution
Lysates/proteins at 40 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR66-225 overnight at 4°C.
IR66-225 anti-MMP1 antibody_Huh-7 ICC image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR66-225 anti-MMP1 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR66-225 anti-MMP1 antibody_A549 PMA treated ICC image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR66-225 anti-MMP1 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR66-225 anti-MMP1 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse testis labeling MMP1 with IR66-225 at 1/100.