Product Includes
Application Dilution
Immunofluorescence 1:300 – 1:400
Immunohistochemistry (Paraffin) 1:300 – 1:500
Flow Cytometry 1:100 – 1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
| Title | |
|---|---|
| No data. | |
Reviews
IR252-897 anti-CD3 antibody WB image
Anti-CD3 antibody at 1/500 dilution
Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with IR252-897 overnight at 4°C.
IR252-897 anti-CD3 antibody FACS image
Product：CD3 antibody
Catalog：IR252-897
Cell: Human peripheral blood
CD3 antibody: 1-100
IR252-897 anti-CD3 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD3 with IR252-897 at 1/400.
IR252-897 anti-CD3 antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min, permeabilized with 0.1% NP-40 for 10 min then blocked with 5% BSA for 30 min. IR252-897 anti- CD3 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR252-897 anti-CD3 antibody WB image
Anti-CD3 antibody at 1/2000 dilution Lysates at 30 μg per lane. This blot was produced using a 15% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with IR252-897 overnight at 4°C.